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排序方式: 共有207条查询结果,搜索用时 15 毫秒
61.
Yamasaki M Kitagawa T Chujo H Koyanagi N Nishida E Nakaya M Yoshimi K Maeda H Nou S Iwata T Ogita K Tachibana H Yamada K 《Journal of agricultural and food chemistry》2004,52(11):3644-3648
Previous studies have shown the physiological significance of dietary conjugated linoleic acid (CLA) in various experimental animals and in human beings. One of the important problems to better elucidate is the difference between triglyceride (TG) and free (FFA) dietary CLA. Here, using splenocytes, this study assesses how TG- and FFA-CLA modulate immunoglobulin and various cytokine productions. In this study, C57BL/6N mice were fed an experimental diet containing 0% CLA, 0.1 or 1% FFA-CLA, or 0.1 or 1% TG-CLA for 3 weeks. The production of immunoglobulin tended to be up-regulated by 1% FFA-CLA. As a result of protein array analysis using the supernatant from splenocytes cultured with no CLA, 1% FFA-CLA, and TG-CLA, some cytokine production was shown to be remarkably regulated by dietary FFA- and TG-CLA. A total of 32 cytokines were examined, and 11-14 produced cytokines that were 2-fold up-regulated as compared with control for FFA- or TG-CLA, respectively. Especially, the production of IL-9 and MCP-5 and other cytokines was remarkably up-regulated by both FFA- and TG-CLA. In addition, seven cytokines were 2-fold down-regulated by TG-CLA. These data show that there is a slight but significant difference between the functionalities of FFA- and TG-CLA. 相似文献
62.
Molecular and morphological variation of six perennial and five annual species including domesticated chickpea, C. arietinum, were inferred on the basis of RAPD and S.E.M. seed coat features using three outgroup taxa (Lens ervoides, Lathyrus japonica and Pisum sativum). Of the 66 decamer arbitrary primers tested, eight primers revealed 87 informative fragments. Neighbor-joining cluster analysis using Jaccard's coefficient of similarity on the basis of polymorphic fragments indicated a narrow variation in C. arietinum and recognized two major clusters in the genus Cicer. The first one included four species of sect. Monocicer: C. echinospermum, C. arietinum, C. reticulatum and Iranian material of C. bijugum. The second cluster contained annual and perennial species belonging to sections Chamaecicer, Polycicer and Acanthocicer. The character state of morphological and ecological traits on the RAPD phenogram indicated no monophyletic incision. Our results suggested that the high genetic difference between annual and perennial species might be regarded as a rapid speciation of section Monocicer. Reconsideration of traditional classification of sections Polycicer and Acanthocicer is necessary. The Desi and Kabuli types of C. arietinum could not be separately grouped at the DNA level, and the low level of genetic variation of C. arietinum may result from a bottleneck during the domestication process. 相似文献
63.
Yamada K Hirotsu T Matsuki M Butcher RA Tomioka M Ishihara T Clardy J Kunitomo H Iino Y 《Science (New York, N.Y.)》2010,329(5999):1647-1650
Population density-dependent dispersal is a well-characterized strategy of animal behavior in which dispersal rate increases when population density is higher. Caenorhabditis elegans shows positive chemotaxis to a set of odorants, but the chemotaxis switches from attraction to dispersal after prolonged exposure to the odorants. We show here that this plasticity of olfactory behavior is dependent on population density and that this regulation is mediated by pheromonal signaling. We show that a peptide, suppressor of NEP-2 (SNET-1), negatively regulates olfactory plasticity and that its expression is down-regulated by the pheromone. NEP-2, a homolog of the extracellular peptidase neprilysin, antagonizes SNET-1, and this function is essential for olfactory plasticity. These results suggest that population density information is transmitted through the external pheromone and endogenous peptide signaling to modulate chemotactic behavior. 相似文献
64.
65.
Hemolytic suppression roles of taurine in yellowtail Seriola quinqueradiata fed non-fishmeal diet based on soybean protein 总被引:1,自引:0,他引:1
Shusaku Takagi Hisashi Murata Takanobu Goto Masahiro Hayashi Hideo Hatate Makoto Endo Hirofumi Yamashita Masaharu Ukawa 《Fisheries Science》2006,72(3):546-555
ABSTRACT: To elucidate the hemolytic suppression roles of taurine and the necessity of dietary taurine supplementation in yellowtail Seriola quinqueradiata fed a diet without fishmeal, juvenile fish with an initial body weight of 250 g were fed for 40 weeks in floating net cages on soybean protein diets supplemented with 0, 3.0, 4.5 and 6.0% taurine. Taurine concentration of the experimental diets were 0.03, 33.9, 52.8 and 71.6 mg/g, respectively. On the 21st week, fish fed the taurine unsupplemented diet had inferior growth and feed performances, higher death, and there were incidences of green liver and hemolytic anemia. In this group, hepatic and plasma taurine concentrations, serum osmolality and osmotic tolerance of erythrocytes (EC50 value) were significantly lower, and plasma hydroperoxide concentration was markedly higher than in the taurine supplemented groups. These conditions markedly improved corresponding with the increase of dietary taurine concentration. These results indicate that taurine plays a role in hemolytic suppression through osmoregulation and biomembrane stabilization in fish. In addition, it is suggested that yellowtail requires dietary taurine as an essential nutrition for maintaining physiological condition normally. 相似文献
66.
Yoshifumi Sawada Manabu Hattori Masahide Iteya Yasuaki Takagi Kazuhiro Ura Manabu Seoka Keitaro Kato Michio Kurata Hirofumi Mitatake Shigekazu Katayama Hidemi Kumai 《Fisheries Science》2006,72(2):364-372
ABSTRACT: Artificially hatched Seriola species have the problem of malformation, mainly in their vertebrae, head, and mouth parts. To clarify the cause of vertebral malformation, the effects of hypoxia during embryogenesis on the induction of centrum defects was investigated in artificially hatched amberjack Seriola dumerili . Firstly, 7-somite stage embryos were exposed to waters of 0, 12.5, 25, 50, and 100% dissolved oxygen (DO) for 0.5, 1, 2, 3, 4, and 5 h to confirm the effective dose (DO concentration and duration of exposure) of hypoxia that induces somitic disturbances in newly hatched larvae. Exposure of embryos to 12.5% DO concentration for longer than 0.5 h induced somitic disturbances. Following this result, centrum defects in juveniles were investigated by an induction experiment with embryos exposed to 12.5% DO for 2 h at the gastrula, 1- or 2-somite, 10-somite, 15-somite, or heart beating stage. This experiment revealed that centrum defects were induced only during somitogenesis, and somitic disturbances were the premonitory symptom of centrum defects. These results indicate hypoxia during somitogenesis as a possible cause of centrum defects in amberjack. 相似文献
67.
Murashita Koji Takakuwa Fumiaki Matsunari Hiroyuki Yoshinaga Hazuki Yamamoto Takeshi Oku Hiromi Furuita Hirofumi 《Fish physiology and biochemistry》2022,48(4):939-954
Fish Physiology and Biochemistry - To reveal direct effects of various protein sources on digestive physiology of red seabream, Pagrus major (38.5 ± 0.4 g), six different... 相似文献
68.
H. Furuita T. Yamamoto T. Shima N. Suzuki T. Takeuchi 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):725-735
This study investigated the effect of dietary arachidonic acid (AA) in broodstock of Japanese flounder on subsequent egg and larval quality. Diets with similar proximate composition and n-3 HUFA level, but with different AA levels (0.1%, 0.6% and 1.2% of diet), were fed to the broodstock from 3 months before and during the spawning season. Spawning was observed from March to May. Total egg production over the spawning season was highest in fish fed the 0.6% AA diet and lowest in fish fed the 1.2% AA diet. All parameters measured as egg quality (percentage of buoyant eggs, hatching rate, larval survival and normality of larvae.) were highest in fish fed the 0.6% AA diet. AA content in eggs proportionally increased with the dietary AA level. EPA content of polar lipids of eggs had a negative correlation with the AA level in diets whereas the DHA content was independent of dietary AA. The results of this study indicate that a supplement of AA at 0.6 g/100 g diet improved the reproductive performance of Japanese flounder, but a higher level of AA (1.2 g/100 g diet) negatively affected both egg and larval quality due to a potential inhibitory effect on EPA bioconversion. 相似文献
69.
70.
Halil Kurban Hirofumi Saneoka Kunito Nehira Rahmutulla Adilla Kounosuke Fujita 《Soil Science and Plant Nutrition》2013,59(4):589-597
The response of two leguminous plants Alhagi pseudoalhagi and Vigna radiata to seawater salinity was studied over a period of 30 d. The growth of Vigna radiata was markedly and gradually reduced by increasing salinity levels, whereas that of Alhagi pseudoalhagi was promoted at 9.1 and 16.2 dS m-1 salinity but then was slightly reduced at 28.2 dS m-1 salinity. These results indicate that Alhagi pseudoalhagi belongs to the group of halophytic plants. Seawater salinity caused changes in the membrane permeability measured as electrolyte leakage in both plants. Alhagi pseudoalhagi maintained a lower membrane permeability than Vigna radiata. With increasing salinity levels, the membrane permeability decreased in Alhagi pseudoalhagi, whereas, in Vigna radiata it slightly increased at 9.1 dS m-1. The leaf water potential and the osmotic potential decreased in both plants along with the seawater salinity levels. However, the turgor potential and osmotic adjustment in Alhagi pseudoalhagi were maintained at a higher level than in Vigna radiata. The contributions of organic and inorganic solutes to the osmotic adjustment differed: Alhagi pseudoalhagi achieved osmotic adjustment through Cl- and Na+ uptake from the substrate, while the contribution of K+, Ca2+, and organic solutes to the osmotic adjustment was limited. These results suggest that the differences in salt tolerance between Alhagi pseudoalhagi and Vigna radiata can not be due to differences in specific-ion effects, but may be related to some factors involved in membrane permeability and osmotic adjustment. 相似文献